Constructing and expressing acyl-homoserine lactone lactonase (aiiA) gene for enhancing Solanum tuberosum resistance against soft rot disease

Abstract. Suryanti E, Rusamana I, Wahyudi AT, Akhdiya A, Sukamdjaja D. 2022. Constructing and expressing acyl-homoserine lactone lactonase (aiiA) gene for enhancing Solanum tuberosum resistance against soft rot disease. Biodiversitas 23: 1780-1787. Expression of the genes associated with pectinase production in disease Dickeya spp. are regulated by quorum sensing mechanism using acyl homoserine lactone(AHL). Degradation AHL can be developed to reduce Dickeyaspp.The aiiA encodes from Bacillus This study aims construct binary vector inserted (pCAB10) cereus B10 express potato Medians cultivar genome. The aiiAgene was cloned into transformed Agrobacterium tumefaciens LB4404. Agrobacterium-mediated genetic transformation conducted co-cultivation technique.The transgenic plant confirmed through PCR andselected kanamycin dadantii A3. result showed that successfully integrated top CAB10 introduced A. percentage pCAB10 57.6%, while there generation efficiency 11.9% 50 mg/L acetosyringone addition. Four exhibited significantly higher than non-transgenic plants. Mahl1 clone had highest incidence as 7.1% suppression 92.85%.